METHOD FOR IDENTIFYING CULTURE MUTATION OF PLANT AND POLYNUCLEOTIDE FOR IDENTIFYING CULTURE MUTATION OF PLANT

摘要

PROBLEM TO BE SOLVED: To provide a method for identifying culture mutation of a plant in which mutation of genes generated by tissue culture of Ipomoea batatas and its relative wild species is detected by level of DNA and to provide a polynucleotide used for detection of such mutation.;SOLUTION: It was found that a sequence is transferred by culture in retrotransposon existing in a genome of Ipomoea batatas. Then, the retrotransposon sequence having the transfer function was combined with an SSAP method for efficiently detecting a place inserted into the genome in a specific DNA sequence. As a result, the method for identifying culture mutation of Ipomoea batatas and its relative wild species preserved as its genetic resource in which a plant body by which mutation occurred in a stage of tissue culture can efficiently and surely be detected was obtained.;COPYRIGHT: (C)2005,JPO&NCIPI