RECOMBINANT HUMAN CELL AND, METHOD FOR EVALUATING AT LEAST EITHOR ONE OF INDUCTION OF HUMAN DRUG METABOLIZING ENZYME AND DRUG METABOLISM BY USING THE RECOMBINANT HUMAN CELL

摘要

PROBLEM TO BE SOLVED: To provide recombinant human cells for sensitively evaluating both of drug metabolism and drug metabolism enzyme and provide the evaluation method using the recombinant human cells.;SOLUTION: The recombinant human cells are those used for evaluating at least one of the induction of the drug metabolizing enzyme and the drug metabolism and comprises a human drug metabolism enzyme code sequence, a human drug metabolite promoter sequence, a reporter protein code sequence and a bicistronic control sequence. The human drug metabolizing enzyme code sequence, the bicistronic control sequence and the reporter protein code sequence are positioned on the downstream of the human drug promoter sequence. Further, the human drug metabolizing enzyme is transformed by the transcription activity of the promoter, and the reporter protein is transformed with a bicistronic expressing vector.;COPYRIGHT: (C)2005,JPO&NCIPI