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Method for extraction, purification and enzymatic modification of soybean 7S globulin α 'subunit for use as a blood cholesterol lowering agent
Method for extraction, purification and enzymatic modification of soybean 7S globulin α 'subunit for use as a blood cholesterol lowering agent
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机译:用作降胆固醇剂的大豆7S球蛋白α'亚基的提取,纯化和酶促修饰的方法
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摘要
A process for the extraction, purification and enzymatic modification of -conglycinin subunit, wherein -conglycinin is selectively extracted from ground, defatted soy, then precipitated by treatment with aqueous ethanol; the enriched fraction is then subjected to Metal Affinity Chromatography (MAC) in denaturant conditions to obtain the subunit, which is treated with chymotrypsin, then subjected to a further MAC to recover the amino-terminal region of this polypeptide (MW 28,000 Da).
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