Method for detecting the interaction between the substance and the protein, a screening method for proteins that interact with matter, and, materials and method of forming a complex with the substance interacting with the protein

摘要

Comprising detecting the complex is contacted with a play, a protein bait and, formed by the contact, In the method of detecting interaction between a bait and a prey, a protein that can be used as a detectable label, the play By the fusion protein, the bait to have a modification for separation, in a cell-free translation system, the presence of the bait, and performs the translation of the mRNA encoding the fusion protein comprising said play, and interaction Translation The coupling step, thereby generating the play cell-free translation system, brought into contact with the bait and prey, by using the label for detection and the modification for separation, in vitro detection of the complex in (in vitro), I performed a sensitivity analysis of interactions such as protein-protein - protein-protein, nucleic acids. Alternatively, if the bait is a protein, in a cell-free co-translation system, the translation of the mRNA encoding the fusion protein containing the bait, by translation of the mRNA and encoding the fusion protein or proteins including the play, the mutual translation The coupling process of the action, caused to interact to generate the bait and prey, by using the label for detection and the modification for separation, in vitro in the (in vitro), protein-protein, the detection of the complex nucleic acid - protein-protein I performed a sensitivity analysis of interactions such.