The present invention relates to the use as catalysts or variants thereof lipase or esterase in insects biotransformation methods. The present invention may have application in any manner, including hydrolysis, esterification, transesterification, or acylation reaction between an ester transesterification. The present invention also has application in the enzymatic resolution of the compound to produce an optically active compound, in particular, have application to a substrate having a hydrophobic moiety such as fatty acid esters and pyrethroids (However, this but are not limited to).
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