To produce a nano-structure comprising an assembly unit at least one is a connecting element binding fragment of one binding or a derivative thereof, or at least pilin protein. Containing pilin assembly unit may include structural elements and obtained a pilin induction coupling two elements, or both functional elements. It is suitably prepared using assembly methods stepwise, in this method, the nanostructure intermediate having unbound at least one element, the nanostructures are contacted with the assembly unit having coupling elements of different. Any of the coupling element of the assembly unit, with themselves, connecting elements of another unit with the same assembly unable to interact. However, one of the coupling elements, such that, the assembly unit is non-covalently bound to the nanostructure intermediate to interact with unbound joining element of the nanostructure intermediate.
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