Methods for determining sequence variations in a genome-wide associated phenotype

摘要

In a manner that does not use the hypothesis, the present invention provides methods for determining sequence variations in a genome-wide associated phenotype of the seed. In the method of the present invention, and is manufactured by a restriction fragment of a set, to digest the nucleic acid from the individual with the different restriction enzymes one or more for each of the subset of individuals with phenotype. Then, restriction sequences set of tags is determined from restriction fragments Such a set for an individual. Limiting sequence tags for subpopulations of organisms are compared, are grouped into groups of one or more groups each containing restriction sequence tags that contain sequences homologous. The restriction sequence tag group of one or more of the obtained sequence changes associated phenotype are identified. For example, the method of the present invention can be to identify subtle genetic risk factors, is used to analyze the sequence variants of a very large number in patients many samples.