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Region unique rearrangement was used, high productivity mechanism for rearrangement virus production
Region unique rearrangement was used, high productivity mechanism for rearrangement virus production
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机译:使用了区域独特的重排,高效率的机制可以重排病毒
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摘要
Preparation method of the rearrangement virus which uses the region unique rearrangement with in vitro is disclosed. With this invention, annular virus genome DNA is digested with the restriction enzyme, the linear virus genome DNA which adjoins by the region unique rearrangement region is formed, region unique rearrangement is done making use of the genome material of the desire which adjoins in vitro, by the region unique rearrangement region. According to this invention, the further process such as election of rearrangement virus genome DNA of desire losing the blend which the aforementioned region unique rearrangement is done, because can apply to the landlord, it can obtain the many rearrangement virus simultaneously more quickly. Therefore, this invention, several hundred or, prepares several thousand rearrangement viruses, it is possible to apply, to the high productivity mechanism in order the screening to do.
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