The use of these complexes for recombinant methods and generating a double-stranded DNA containing a 3 'single-stranded portion

摘要

The present invention provides a method of recombining nucleic acid. In particular, 'in the process method for the generation of double-stranded nucleic acid, and recombinant polynucleotides partially including a protruding, 3 from the RNA target 3' from the RNA target duplex in part that includes a projection the use of a nucleic acid strand, is described. These methods do not require thermocycling. The present invention allows for the identification of proteins with desired properties or improved properties, also selection methods and recombinant methods, is provided.