Prescribing peptide and the Freund Freund's complete adjuvant which the hypodermic of the manufacturing method null mouse of the hybridoma which produces the monoclonal antibody which

摘要

PROBLEM TO BE SOLVED: To provide a method for producing a hybridoma generating a monoclonal antibody specifically binding to a fragment of human or bovine lactoferrin.;SOLUTION: A monoclonalized hybridoma is obtained by immunizing a mouse through subcutaneous administration of a peptide consisting of an amino acid sequence represented by SEQ ID NO. 2 and Freund's complete adjuvant, extracting spleen on the third day after final immunization, fusing a spleen cell and myeloma cell obtained from the extracted spleen by adding polyethylene glycol, selecting from the fused cells a hybridoma generating an antibody binding to a peptide consisting of an amino acid sequence represented by SEQ ID NO. 1 and performing a limiting dilution technique.;COPYRIGHT: (C)2005,JPO&NCIPI