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Detection of calcineurin isoforms

机译:钙调神经磷酸酶同工型的检测

摘要

A method for detecting isoforms and/or splice variants of Calcineurin or its subunits is established. The inventive method is especially useful for differentiation between the various isoforms of Calcineurin. Herein a polymerase chain reaction of nucleic acids of mammalian tissue is performed and the corresponding products are evaluated. Especially prefered is a reverse transcribed polymerase chain reaction. For the polymerase chain reaction primers for the isoforms and/or splice variants of Calcineurin are used. In a prefered embodiment isoforms and/or splice variants of the catalytic subunit Calcineurin-A are detected.
机译:建立了一种检测钙调神经磷酸酶或其亚基的同工型和/或剪接变体的方法。本发明的方法对于区分钙调神经磷酸酶的各种同工型特别有用。在此,进行哺乳动物组织的核酸的聚合酶链反应,并评价相应的产物。特别优选的是逆转录聚合酶链反应。对于聚合酶链反应,使用钙调磷酸酶的同工型和/或剪接变体的引物。在一个优选的实施方案中,检测催化亚单位钙调神经磷酸酶-A的同工型和/或剪接变体。

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