DNA testing and method of fluorescence detection method and confocal detection method and multi-spot beam forming method

摘要

PROBLEM TO BE SOLVED: To increase the speed and accuracy of detecting a common focus using multi-spot light beams, detecting fluorescence, and performing detection for DNA examination by obtaining from a laser beam proximate multi-spot light beams having little difference in intensity and allowing a high efficiency for utilizing light.;SOLUTION: A plurality (N) of proximate beams are incident on polarization elements for doubling each beam, and the beams are each divided into two according to polarization to provide 2N beams. The polarization elements are used by M to provide 2MN beams. The incident beams are formed into converging beams and an array of pinhole openings is provided in a converging position. 70 or 90% of the energy of a source of laser beams can then be utilized for the multi-spot light beams. Variation in intensity between the spots of the multi- spot light beams thus formed can be held within ±20% or ±10%. The multi- spot light beams thus formed are used in the detection of the common focus and fluorescence and in DNA examination to permit detection and examination at high speed and accuracy.;COPYRIGHT: (C)2001,JPO