The monoclonal antibody cancer-related genes mina53, proteins and Mina53

摘要

Myc protein is a ubiquitous mediator of cell proliferation, I activate gene expression through E- box. If you are encoding a protein with a molecular weight of 53kDa, mina53 gene is present in the nucleolus and in the nucleoplasm. When the cells in the stationary phase is activated by serum, following the increase in the levels of c-myc mRNA, mina53mRNA increased. When in HL60 cells in human promyelocytic leukemia, the expression of c-myc were decreased by TPA, protein mina53mRNA decreased. Instead of mutant c-Myc lacking the activation domain, ectopic expression of wild-type c-Myc was increased expression of mina53. Protein expression and mina53mRNA can also be induced by artificial introduction of the activity of c-Myc. In addition, when the c-Myc of c-MycER chimeric protein during activated, mina53mRNA increases inhibitor on protein synthesis even in the presence of. On the other hand, there is a box E- site close to the transcription start site of the gene mina53, expression from mina53 promoter is activated by c-Myc via E- box. rather than after TPA treatment, c-Myc protein is bound to the promoter region mina53 vivo in HL60 growth phase in (in vivo). Moreover, specific inhibition of mina53 expression in rat 3Y1 cells fibroblasts having a HeLa cells, the c-myc with high expression significantly inhibits cell proliferation. Combining these results, mina53 is a Myc target gene, and shows the relationship to cell proliferation in mammals. Also, it is possible to specifically recognize the protein Mina53 cancer cells, is expressed in tissues or cells of esophageal cancer and colon cancer in particular, anti Mina53 monoclonal antibodies, used for the diagnosis of cancer diseases can be.