With fluorophotometry manner and the fluorophotometry

摘要

PROBLEM TO BE SOLVED: To use a continuous oscillation laser for an excitation light source and easily achieve accurate detection by moving a sample where excitation light from the excitation light source is applied on the light axis of a fluorescence detection means for detecting the incident fluorescent light. ;SOLUTION: A circular biochip 10 where a number of sample spots are arranged is rotated and driven in a direction marked by an arrow R by a rotary motor. A continuous oscillation (CW) laser 30 that is an excitation light source and the optical head of optical systems 40 and 50 for applying the excitation light and detecting fluorescence is placed at the upper portion of the biochip 10. Then, excitation light from the CW laser 30 is applied to one sample spot 11a on the rotating biochip 10 via an excitation application optical system 40 to excite a fluorescent substance in a sample spot 11a. After that, the sample spot 11a where the excitation light is applied is moved along with the rotation of the biochip 10 and reaches immediately below the fluorescence detection optical system 50. Fluorescence being generated from the sample spot 10a at this position enters the fluorescence detection optical system 50 for detection.;COPYRIGHT: (C)2000,JPO