The liquid constituent for glass conversion retention of embryo and the super low temperature preservation method null (1)10 (v/v)

摘要

PROBLEM TO BE SOLVED: To provide a liquid composition for vitrifying preservation of embryos so as to establish the practical vitrifying preservation, wherein the composition is capable of preserving porcine early blastocyst covered with a perfect zona pellucida also applicable to disease control thereof, and to provide a simple and highly reproducible ultra-low temperature preservation method for porcine embryos.;SOLUTION: This liquid composition for the vitrifying preservation for porcine early blastocyst is characterized by comprising (1) an M2 liquid added with v/v 10% ethylene glycol, (2) an M2 liquid added with v/v 10% ethylene glycol, 0.6 mol sucrose and w/v 1% polyethylene glycol and (3) an M2 liquid added with v/v 40% ethylene glycol, 0.6 mol sucrose and w/v 2% polyethylene glycol. The other objective ultra-low temperature preservation method for porcine embryos comprises using the composition.;COPYRIGHT: (C)2002,JPO