Its use and the three-dimensional structure of DNA recombination repair enzyme

摘要

And determines the three-dimensional structure to produce a crystal of a protein Rad52 is a recombinant DNA repair enzymes, subjected to X-ray crystal structure analysis by using the same. Three-dimensional structure of the Rad52 protein complex obtained in this way can be used to screen for compounds binding to the enzyme complex and mutant enzymes variety. Compounds that modulate the recombinant DNA repair capacity by binding to the complex and mutant Rad52 protein complex were screened by the method of the present invention, by the diagnosis or treatment of diseases caused by DNA damage and Rad52 in vivo In addition it can be used to detect DNA recombination repair capacity, and may be used, for example, gene transfer to specific sites by homologous recombination in vivo and Drug Discovery targeting Rad52.