Three-dimensional structure of DNA recombination/repair protein and use thereof

摘要

A crystal of DNA recombination/repair protein, Rad52 protein is prepared and the three-dimensional structure thereof is determined by X-ray crystallography with the use of the same. The obtained three-dimensional structure of Rad52 protein can be used for screening a variety of mutant enzymes or compounds which binds to the enzyme complex. The mutant Rad52 protein complex, or the compound that binds to the complex so as to modulate the DNA recombinational repairing activity, which are screened by the method of the present invention, can be used for diagnosis and treatment of diseases induced by DNA damages, and detection of the DNA recombinational repairing activity in vivo. Moreover, these substances are usable in drug discovery targeting to Rad52, and gene transfer to a specific site by homologous recombination, etc.