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Methods and compositions for altering the functional properties of seed storage proteins in soybean

机译:改变大豆种子贮藏蛋白功能特性的方法和组合物

摘要

The present invention provides methods and compositions useful for altering the functional properties of soybean seed storage proteins. It is the novel finding of the present invention that the functional properties of seed storage proteins can be altered by reducing the expression of one or more vacuolar processing enzymes in plant seed. Accordingly, in one embodiment, the invention provides a method for altering the functional properties of one or more soybean seed storage proteins. The method comprises transforming a soybean plant cell with at least one expression cassette capable of expressing a polynucleotide that reduces the activity of a vacuolar processing enzyme in the seed of said soybean plant, regenerating a transformed plant from the transformed plant cell, and collecting seed from the regenerated transformed plant. Plants that are genetically modified or mutagenized to alter the functional properties of one or more seed storage proteins, and the transgenic seed of such plants are also provided.
机译:本发明提供了可用于改变大豆种子贮藏蛋白的功能特性的方法和组合物。本发明的新发现是,可以通过减少植物种子中一种或多种液泡加工酶的表达来改变种子贮藏蛋白的功能特性。因此,在一个实施方案中,本发明提供了一种改变一种或多种大豆种子贮藏蛋白的功能特性的方法。该方法包括用至少一个表达盒转化大豆植物细胞,该表达盒能够表达降低所述大豆植物种子中的液泡加工酶活性的多核苷酸,从所述转化的植物细胞再生转化的植物,以及从再生的转化植物。还提供了经过遗传修饰或诱变以改变一种或多种种子贮藏蛋白的功能特性的植物,以及此类植物的转基因种子。

著录项

  • 公开/公告号US2005155102A1

    专利类型

  • 公开/公告日2005-07-14

    原文格式PDF

  • 申请/专利权人 DARREN B. GRUIS;RUDOLF JUNG;

    申请/专利号US20040011522

  • 发明设计人 DARREN B. GRUIS;RUDOLF JUNG;

    申请日2004-12-14

  • 分类号A01H1/00;C12N15/82;A01H5/00;

  • 国家 US

  • 入库时间 2022-08-21 22:25:11

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