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Making and using microclonal uncloned cDNA libraries
Making and using microclonal uncloned cDNA libraries
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机译:制备和使用微克隆未克隆的cDNA文库
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摘要
A method is described for the preparation, characterization and utilization of temporally ordered panels of cDNA libraries from human microclones. Each microclone undergoing morphogenetic differentiation in vitro differs in discrete gene expression. The developmental stage of the microclone is determined and the gene expression patterns of the microclones temporally ordered and arranged into expression profiles and temporal spectra. Direct comparison of the cDNAs produced from different neural microclones allows identification of novel RNA transcripts.
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