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Nucleic acids, compositions, methods, and kits for detecting mycoplasma and acholeplasma species

机译：用于检测支原体和无形体物种的核酸，组合物，方法和试剂盒

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摘要

The invention relates to nucleic acids, methods, compositions, and kits for the PCR-based detection of Mycoplasma and Acholeplasma bacterial species. The nucleic acids, methods, compositions, and kits provide for increased specificity and sensitivity of PCR-based Mycoplasma bacterial assays. Primer sets and PCR-based assays are provided that amplify and detect conserved 16S rRNA gene sequences from multiple Mycoplasma and Acholeplasma species while avoiding amplification and detection of non-target sequences.

机译：本发明涉及核酸，方法，组合物和试剂盒，用于基于PCR的支原体和霍乱质子细菌种类的检测。核酸，方法，组合物和试剂盒可提高基于PCR的支原体细菌检测的特异性和敏感性。提供了引物组和基于PCR的检测方法，可扩增和检测来自多个支原体和 Acholeplasma 物种的保守16S rRNA基因序列，同时避免了扩增和检测非靶序列。 展开▼

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公开/公告号US2005250112A1

专利类型

公开/公告日2005-11-10

原文格式PDF

申请/专利权人 GOTHAMI A. PADMABANDU;FATEMEH SALEHI;PETER LOUIS PINGERELLI;REINHOLD DIETRICH MUELLER;
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申请/专利号US20040840672

发明设计人 FATEMEH SALEHI;GOTHAMI A. PADMABANDU;REINHOLD DIETRICH MUELLER;PETER LOUIS PINGERELLI;
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申请日2004-05-07

分类号C12Q1/68;C07H21/04;

国家 US

入库时间 2022-08-21 22:21:40

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