Method for the treatment of diseased, degenerated, or damaged tissue using three dimensional tissue produced in vitro in combination with tissue cells and/or exogenic factors

摘要

The invention relates to a tissue replacement structure comprising (a) a preformed three-dimensional tissue which can be produced by obtaining cells from a human or animal organism and culturing them in a stationary fashion as a suspension culture in cell culture vessels with hydrophobic surface and tapering bottom until a cell aggregate is formed which has differentiated cells embedded therein and has an outer region wherein cells capable of proliferation and migration are present; (b) (i) an autologous cell suspension which can be produced from endogenous cells, with endogenous serum being added, with no addition of growth-promoting compounds, (ii) implants or support materials and/or (iii) growth factors; and/or (c) can be obtained by exposure of the tissue according to (a) to electromagnetic fields, mechanical stimulation and/or ultrasound.