Process for obtaining transgenic leguminous plants (LEGUMINOSAE) containing exogenous DNA

摘要

The present invention refers to a plant produced by a process of selecting germ line-transformed leguminous plants. The process includes introducing exogenous genes into apical meristematic cells of the embryonic axis of leguminous plants by bombarding the plant cells with a DNA construct which includes a sequence which encodes a protein capable of conferring tolerance to a herbicide selected from the group consisting of an imidazolinone and a glyphosate, inducing multiple shoot formation from the transformed cells by culturing the embryonic axis in a medium containing a cytokinin, and selecting the meristematic cell-derived shoots obtained by culturing the embryonic axis on a medium containing imidazolinone or glyphosate.