Methods to identify if a compound or an antibody inhibits the entry of a vu00ecrus in a cell, a receiver superfu00eccie cell and a mutation in a vu00ecrus that confers resistance to a compound.To produce a composition and to determine the susceptibility and resistance of a composite vu00ecrus

摘要

Methods to identify if a compound or an antibody inhibits the entry of a vu00ecrus in a cell, a receiver superfu00eccie cell and a mutation in a vu00ecrus that confers resistance to a compound.To produce a composition and to determine the susceptibility and resistance of a vu00ecrus a composed ".The invention provides a method to identify a compound inhibits the entry of a virus in a cell which comprises: (a) obtaining nucleic acid encodes a protein of the viral envelope from aPatient infected by the virus, (b) Co transfectar in a first cell (I) the nucleic acid of step (a),And (II) a viral vector expression in that they lack a nucleic acid coding for a protein of the envelope, and which comprises a nucleic acid indicator that produces a detectable signal.So the first cell produces viral particles comprising the envelope protein encoded by the nucleic acid obtained from the patient.(c) contacting with the viral particles produced in step (b) with a second cell in the presence of the compound.In the second cell expresses a cell surface receptor that the virus is bound; (d) to measure the quantity of the signal produced by the second cell in order to determine the infectivity of the pairTu00edculas viral, and (E) to compare the amount of signal measured in step (d) with the amount of signal produced in the absence of compound.When a small amount of signal measured in the presence of the compound indicates that the compound inhibits the entry of the virus in the second cell.