Oligonucleotu00ecdeos, use and method for typing, the determination of viral dominance and quantification on the human papilomavu00ecrus using a combination of molecular techniques.Oligonucleotu00ecdeos and compounds.

摘要

"Oligonucleotu00ecdeos, use and method for typing, the determination of viral dominance and quantification on the human papilomavu00ecrus using a combination of molecular techniques.Oligonucleotu00ecdeos and compounds ".The present invention relates to the combined use of molecular biology techniques.Specifically, the nested multiplex PCR technology (polymerase chain reaction doubled) with general consensus oligonucleotides followed by multiple pairs of oligonucleotides specific and COmponentes (reagents and reaction conditions) for tipar the human papillomavirus in biological samples from human tissues, such as cervical swab, scraped the penis, mouth swab,Among others. Originally this invention amplifies at least 40 HPV types in general, then specific 32 types (SEQ.ID numbers 1 to 70) that may potentially be infected samples of human tissue.The procedures include the determination of multiple infections combined with the acquisition of the relationship of the viral load between the HPV types.Also called viral dominance in a particular stage of infection.32 viral types are described as part of a kit of multiplex PCR for simultaneous detection and typing of the virus.The sequ00b3u00eancias primers (specific primers or oligonucleotides) general consensus degenerate (1 a PCR reaction) and combined.As well as the specific primers combined (2 a PCR reaction (nested) for viral types, which include non oncogu00eanicos, oncogu00eanicos and indeterminate; 6, 11, 16, 18, 26, 30, 31, 33, 34, 35.39, 40, 42, 44, 45, 51, 52, 53, 54, 55, 56, 57, 58, 59, 61, 62, 64, 66, 67, 68, 70, mm7, mm8 and mm9.The quantification of viral infections among the types in multiple series of dilutions of DNA template amplification, after the initial general (1 a PCR) is determined.Both the viral dominance during clinical follow-up of the patients.The purpose of the dilution prior to amplification is to obtain a profile of bands of infective viral types.Allowing the determination of the relative proportion between specimens of HPV present in the sample tested, as well as their variations during the progress of infection.