Metabolic engineering for improved saccharomyces cerevisiae xylose utilization

摘要

"METABOLIC ENGINEERING FOR THE USE OF PERFECT XYLOSE OF SACCHAROMYCES CEREVISIAE". The present invention relates to a method for the preparation of an ethanol producing strain of Saccharonias cerevisiae, which optionally utilizes xylose, comprising genes for overexpression of xylose reductase, xylitol dehydrogenase and xylulokinase. In accordance with the present invention, said method is characterized in that in addition to said genes, genes for the production of phosphoacetyl transferase, and acetaldehyde dehydrogenase are introduced and optionally overexpressed. The present invention relates to the use of a recombinant yeast. Also in accordance with the present invention, said use is characterized in that the yeast is prepared in accordance with the method described above for the production of optionally containing xylose ethanol in growth medium, and / or for the production of xylose-containing ethanol in growth medium.