Bioinsecticide based on a Strain of Bacillus thuringiensis (Berliner), formulacvion and Production procedures

摘要

A Biological control Agent, characterized in that it comprises a native Strain of Bacillus thuringiensis deposited under Registration Number 202194 ATCC strain was characterized using the technique of polymerase chain reaction (PCR), and which is highly toxic to Insect L Order Lepidoptera whose Scientific name is Spodoptera frugiperda.A Biological control Agent according to claim 1 in which the strain produces a Protein of 130 to 135 kilodaltons (KDA). A Biological control Agent according to claim 2, used as the active ingredient contained in a Crystal Protein produced by this Strain.A Biological control Agent in accordance with claims 1 to 3, which is the active ingredient that produces spores that Strain.The Biological control Agent according to claims 1 to 4, which is characterized by having a median Lethal Concentration (Lc 50) for Insect Spodoptera frugiperda between 80 and 160 nanograms (NG) of toxic Protein per square Centimeter of Artificial Diet in terms of Laboratory.A process for the industrial production of a Biological control Agent to treat claims 1 to 5, which contains Culture Media with combinations of components to achieve a final concentration between 12 to 15 g / l of Active Ingredient, with insecticidal activity Against Spodoptera frugiperda between 45 - 87.5% mortality against a standard of Lethal dose 50, which is fermentation, which comprises the steps of: preparing a inoculum which corresponds to 10% of the volume of the inoculum the inoculum to activate from a Pure Strain of Bacillus thuringiensis Spore and preserved in an environment only Lido - Keep the inoculum for a period not to exceed 16 hours- separate the Culture Medium obtained by Filtration, dried the cake obtained from the active ingredient and a stage of size reduction in a Mill, adding Solvent and / or adjuvant formulation.