Method for identifying and isolating genome fragments with linkage disequilibrium involving the conduction of an early cloning step

摘要

A method for identifying and isolating genome fragments with linkage disequilibrium is described. For related and unrelated individuals, parts of the genome which contain candidate gene parts that are in linkage disequilibrium with their narrow DNA environment are isolated. The cloning step is carried out earlier than in other methods, enabling the DNA fragments to be obtained independently of volume and also replacing the methylation step still carried out in other methods. The use of a plant enzyme considerably increases the specificity of the method compared with methods which use the Mut S,H,L complex. Number 21 indicates the first individual, while 23 represents a second individual, 22 indicates the first individual plus linker, and 24 indicates the second individual plus linker. Number 30 designates the vector. The method steps are denoted E1 to E4 and have the following meanings: E1 is the Eco R1 digestion, E2 is maturation, E3 is the mixing of the first and second individuals with renaturation (PERT), and the method is followed by ring closure.