Method for detecting the formation of complexes biomolecule-ligand non-covalently.

摘要

Procedure for the detection of complex formation biomolecule-ligand non-covalently.; The method comprises the following steps: a) preparing a solution comprising at least one ligand and a biomolecule; b) preparing a control solution comprising at least one non-bondable molecule resulting in a signal strength similar to a ligand or biomolecule of step (a) MALDI; c) analysis of the preparations obtained in steps (a) and (b) by mass spectrometry MALDI-TOF.; Said method requires very small amounts of material, to analyze complex samples, detect a broad mass range and has high reproducibility and sensitivity.