Compounds, methods and equipment to identify agents capable of altering the protein-protein interaction.

摘要

reverse mammalian hybrid dual system for identifying a change in protein-protein interaction comprising: a suppressor construct comprising a constitutive promoter operably linked to a coding sequence repressor protein; an encoding construct interactive fusion protein comprising a promoter operably linked to a repressible operator repressible by a repressor protein encoded by said encoding repressor protein, a first coding sequence interactive protein fused in frame with a coding sequence protein transcriptional activation domain and a second coding sequence fused in frame with interactive protein coding sequence protein DNA binding domain; and, a reporter construct comprising a DNA binding element that can bind to said protein binding domain operably linked DNA to a minimal promoter and a reporter secuenciacodificante.