NEW POLYMER COMPLEXES FOR THE TRANSFECTION OF NUCLEIC ACIDS, WITH RESIDUES THAT DISSTABILIZE CELLULAR MEMBRANES.

摘要

THE INVENTION REFERS TO A COMPLEX BETWEEN AT LEAST A NUCLEIC ACID (NEGATIVELY CHARGED) AND AT LEAST A POSITIVELY CHARGED POLYMERIC CONJUGENT, BEING THE LINK BETWEEN THE NUCLEIC ACID AND THE POLYMERIC CONJUGATE OF UNI-CONNECTED POLYMATE CONNECTIVITY MONOMERAS THAT HAVE NH 3 + FREE FUNCTIONS, AND BEING THAT: THE NH 3 + FUNCTIONS FREE OF SUCH MONOMERAL UNITS ARE REPLACED IN A RELATIONSHIP OF THE LESS THAN 10% BY WASTE THAT DRAG IN THE MIDDLE AVERAGE A DISABLING OF CELLULAR PARARIAL MEMBRANES THE MEMBRANE OF THE VESICLES OF ENDOCITOSIS AND / OR OF THE ENDOSOMAS; HAVING SUCH RESIDUES ALSO THE FOLLOWING PROPERTIES: THESE UNDERSTAND A FUNCTIONAL GROUP THAT ALLOWS YOU TO FIX THAT POLYMER; THEY ARE NOT ACTIVE AS A RECOGNITION SIGNAL RECOGNIZED BY A CELL MEMBRANE RECEIVER; THEY CAN UNDERSTAND AT LEAST ONE FREE NH 3 + FUNCTION; HAVING SUCH UNLOADED WASTE ALSO THE FOLLOWING PROPERTIES: UNDERSTAND AT LEAST A HYDROXYL GROUP; THEY ARE NOT ACTIVE AS A RECOGNITION SIGN RECOGNIZED BY A CELL PHONE MEMEBRANE RECEIVER; BEING ABLE THE HYDROXYL GROUPS OF SUCH UNLOADED WASTE BE REPLACED BY AT LEAST A MOLECULA WHICH CONSTITUTES A RECOGNITION SIGNAL RECOGNIZED BY A CELLULAR MEMBRANE RECEIVER, ALWAYS THE SET OF FUNCTIONS AT NUMBER 30 OF NUMBER 3 OF THE MONOMARY UNITS OF THE POLYMER NETWORK OF DICHO CONJUGADO POLIMERICO.