Method for the generation of genetically modified precursor lymphocytes vertebrates, and use thereof for production of heterologous proteins junction.

摘要

A method for the generation of vertebrate lymphocytes that can be used for the production of any binding protein or a functional fragment thereof with the ability to selectively bind to an antigen or ligand, including any heterologous antibody, any antigen receptor composed of variable domains and constant regions comprising receptors T and immunoglobulins membrane-bound cells, any artificial binding protein displaying immune effector functions wildtype or artificial modified effector functions or not derivable antigen receptor or immunoglobulins heterologous encoded by the germline, and any functional fragment thereof, comprising the steps of: (a) genetically modifying vertebrate precursor lymphocytes, which (i) are derived from primary lymphoid organs, and (ii) have the potential to differentiate into cells of l ine mature lymphoid, by introducing at least one exogenous genetic element encoding at least one binding protein or functional fragment thereof, and (b) effecting differentiation of said cells genetically modified precursor cell line lymphoid mature both in vitro and in vivo, thereby generating lymphocytes capable of producing mode said binding protein or functional fragment thereof, with the proviso that the in vivo production in humans is excluded.