Mono- and poly-recombined antigens for detecting cytomegalovirus specific IgM in human sera by the immunoenzymatic dosed.

摘要

It DESCRIBED a mixture of materials POLIEPITOPES RECOMBINANT PROTEIN MONO AND FULLY CAPABLE OF REPLACING THE VIRAL ANTIGENS A when used in enzyme immunoassay (EIA); The mixture includes a fusion protein POLY-EPITOPE having a first region comprising an amino acid sequence (H10) corresponding to that of the last 233 amino acids from the COOH OF PROTEIN VIRAL P52 OR A PART THEREOF, a second region comprising an amino acid sequence (F3) corresponding to that of the last 43 amino acids from the COOH PROTEIN VIRAL PP150 OR A PART OF IT, and a third region comprising an amino acid sequence (A1C2) corresponding to the taking of AA 595 AA 614, proceeding in the 5 '- 3' ', the same protein VIRAL PP150; And, combined, a second fusion protein includes a sequence of amino acids corresponding to those taken originating in the 5 '- 3' 'AA 297 to AA 510 MAIN PROTEIN PP65 DEMATRIZ VIRAL encoded by the gene VIRAL PROTEIN AND A THIRD UL83 fusion includes a sequence of amino acids corresponding to those taken originating in the 5 '- 3' 'AA AA 373 117 A SIMILARITY OF VIRAL PROTEIN PP38 encoded by the gene VITAL UL80A . THESE THREE fusion proteins can be used COMBINED FOR THE PREPARATION OF AN ELISA TEST KIT FOR SCREENING SPECIFIC IGM Cytomegalovirus in human serum.