A METHOD FOR INCREASING THE EFFICIENCY OF TRANSGENIC ANIMAL PRODUCTION

摘要

This invention discloses a method for increasing prodn. rate of transgenic animals. This method uses somatic nucleus transplantation, including gene-transplatning procedures, to produce transgenic animals. It is characterized by that: simultaneous expression of green fluorescin gene and neomycin resistance gene double-labeling selection carrier is used to achieve transgenic cell and transgenic clon embryo effective selection. This invention establishes a set of methods for effectively increasing the prodn. rate of somatic clone mediated transgenic animals, particularly domestic animals. This invention can be used for producing medicine-protein, nutrient-protein, industrial-protein and for heteroplastic transplantation, and for producing human milk like ox-milk.