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Cell expansion lethal toxin and detection of bacterium belonging to campylobacter genus that targets the same

机译:细胞膨胀致死毒素和靶向弯曲杆菌属的弯曲杆菌属细菌的检测

摘要

The present inventors succeeded in cloning the CDT genes of C. coli and C. fetus, which were previously unknown, and in determining their sequences. In addition, the inventors also developed specific primers and primers common to the two species by comparing the CDTs of C. jejuni and C. fetus. Furthermore, the inventors demonstrated that these primers were applicable to multiplex PCR that simultaneously allows for the rapid and convenient determination of the presence of Campylobacter CDT and identification of species, and that they can also be used in PCR-RFLP-based typing.
机译:本发明人成功克隆了以前未知的大肠杆菌和胎儿梭状芽胞杆菌的CDT基因,并确定了它们的序列。另外,发明人还通过比较空肠弯曲杆菌和胎儿弯曲杆菌的CDT,开发了特异性引物和两个物种共有的引物。此外,发明人证明了这些引物适用于多重PCR,其同时允许快速方便地确定弯曲杆菌CDT的存在和种类的鉴定,并且它们也可以用于基于PCR-RFLP的分型。

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