Cell line comprising the promoter of cyclooxygenase-2 (COX-2) and a witness gene, and use thereof in the search of COX-2 transcriptional induction selective inhibitors

摘要

Cell line is constructed including a DNA, it includes all or part of promoter sequence for the gene of cyclooxygenase 2 (steersman -2) and reporter coding, it is effectively connected to each other, so that the expression of reporter described in the promoter sequence in response to -2 gene regulations of steersman of a suitable stimulus. Assay method includes entering connection with the cell line to be analyzed with compound and determine the presence of the indication signal due to the movable expression of reporter. The method passes through suitable stimuli. in steersman -2A transcriptional level be applicable to inductionimage