VECTOR FOR CLONING AN INSERT HAVING BLUNT ENDS, ENABLING RECOMBINANT CLONES TO BE POSITIVELY SELECTED, METHOD FOR CLONING IN SAID VECTOR, AND USE OF SAID VECTOR FOR EXPRESSING AN INSERT

摘要

The invention relates to a vector for cloning an insert having blunt ends, enabling recombinant clones to be positively selected, to a method for cloning an insert in said vector, and to the use of said cloning vector especially for expressing an insert. The inventive cloning vector comprises a nucleotide sequence coding for a poison protein and a restriction enzyme site located in an essential coding region of said nucleotide sequence, said restriction enzyme generating blunt ends and being active in ligation conditions.