PROCESS FOR PRODUCING MODIFIED Ϝ-GLUTAMYLTRANSPEPTIDASE (MODIFIED GGT) HAVING ENHANCED GLUTARYL-7-AMINOSEPHALOSPORANIC ACID (GL-7-ACA) ACYLASE ACTIVITY

摘要

It is intended to produce a modified peptide having an enhanced glutaryl-7-aminosephalosporanic acid (GL-7-ACA) acylase activity (i.e., a modified GGT) by using Ϝ-glutamyltranspeptidase (GGT) which is an enzyme widely occurring in living organisms. A modified GGT having an enhanced glutaryl-7-aminosephalosporanic acid (GL-7-ACA) acylase activity can be produced by substituting one or more amino acid residues in GGT selected from the group: 1) an amino acid residue interacting with a Ϝ-glutamyl compound; and 2) an amino acid residue in the area round the active center, by other amino acid residue(s).