PROBES FOR DETECTING PROTEIN NUCLEAR MIGRATION AND METHOD OF DETECTING AND QUANTIFYING PROTEIN NUCLEAR MIGRATION USING THE SAME

摘要

A convenient and highly accurate method for detecting protein nuclear migration due to an endogenous or foreign substance in a local area such as a vital cell or an individual organism with the use of a pair of probes for detecting protein nuclear migration, characterized in that the probes comprising a probe I having the protein, the nuclear migration of which is to be detected and quantified, connected to either the N-terminal side or the C-terminal side of a fused structure [intein-C/labeled protein-C] wherein at least a C-terminal side polypeptide obtained by halving intein is connected to a C-terminal side polypeptide obtained by halving a labeled protein in this order, and another probe II having a nuclear localization signal connected to either the N-terminal side or the C-terminal side of a fused structure [labeled protein-N/intein-N] wherein at least the remaining N-terminal side polypeptide obtained by halving intein is connected to the remaining N-terminal side polypeptide obtained by halving the labeled protein in this order.