IN VITRO SYNCHRONISATION OF NUCLEAR AND CYTOPLASMATIC MATURATION OF OOCYTES INVOLVING ADDITION AND REMOVAL OF PHOSPHODIESTERASE TYPE 3 INHIBITOR

摘要

The method for in vitro synchronisation of nuclear and cytoplasmatic maturation of GV oocytes from domestic animals or from primates can be improved if a phosphodiesterase type 3 inhibitor is added to the medium after collection of the oocytes and, thereafter, said phosphodiesterase type 3 inhibitor is removed to allow the nuclear maturation to proceed.