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FLUORESCENCE-QUENCHING USED TO DETECT NUCLEIC ACID OLIGOMER HYBRIDIZATION EVENTS AT HIGH SALT CONCENTRATIONS
FLUORESCENCE-QUENCHING USED TO DETECT NUCLEIC ACID OLIGOMER HYBRIDIZATION EVENTS AT HIGH SALT CONCENTRATIONS
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机译:荧光猝灭检测高盐浓度下的核酸低聚物加氢反应
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摘要
A method is described for detecting nucleic acid oligomer hybridization events by fluorescence quenching, which comprises as a first step the provision of a modified surface. The modification of the surface consists in the binding of at least one type of modified nucleic acid oligomers 201 , wherein said nucleic acid oligomers 201 are modified by at least one type of fluorophore 102 bound to it. The further steps of the inventive method are: providing a sample that includes nucleic acid oligomers, contacting said sample with the modified surface, adjusting a defined salt concentration of greater than 0.5 mol/l in the solution surrounding the modified nucleic acid oligomers, detecting the fluorescence of the fluorophore and comparing the detected fluorescence intensity with reference values.
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