MONOCLONAL ANTIBODY SPECIFIC TO HUMAN CACYBP/SIP BEING USEFUL FOR STUDY ON THE IN VIVO FUNCTION OF HUMAN CACYBP/SIP PROTEIN AND FUSION CELL LINE PRODUCING THE SAME

摘要

PURPOSE: A monoclonal antibody specific to human CacyBP/SIP(Calcyclin and Siah-1-interacting Protein) and a fusion cell line producing the same are provided, which monoclonal antibody is useful for study on the in vivo function of human CacyBP/SIP protein. CONSTITUTION: The monoclonal antibody specific to human CacyBP/SIP(Calcyclin and Siah-1-interacting Protein) is produced by a fusion cell line SIP60 1G3(KCTC 10443BP), and specifically recognizes the amino terminal fragments of the human CacyBP/SIP protein, wherein the subclass type of the monoclonal antibody is IgG1. The method for mass-producing the monoclonal antibody specific to human CacyBP/SIP comprises the steps of: (1) injecting the fusion cell line SIP60 1G3(KCTC 10443BP) into the abdominal cavity of a mouse; (2) collecting the abdominal dropsy from the mouse; and (3) isolating the monoclonal antibody specific to human CacyBP/SIP from the abdominal dropsy.