MUTANT GENE OF DEXTRANSUCRASE PRODUCING ONLY OLIGOSACCHARIDE, RECOMBINANT VECTOR COMPRISING THE MUTANT GENE, MICROORGANISM TRANSFORMED BY THE RECOMBINANT VECTOR, AND THE DEXTRANSUCRASE PRODUCED FROM THE MICROORGANISM

摘要

DNA sequences of the present invention is a modified dextran Klein dehydratase gene, present invention relates to the recombinant vector, the transformed microorganism with such a recombinant vector containing the gene, and the dextran sucrase azepin produced from this microorganism, SEQ ID NO: 1 to be made, sugar used as the substrate does not synthesize dextran when using maltose as the acceptor sugar as a substrate can be modified dextran for synthesizing only the oligosaccharide receptor product Klein dehydratase gene, the modified number of dextran a: (KACC95015 DSRN, deposit number), and the transformed Klein with a recombinant vector, a substrate for the transformed sugar with the recombinant vector containing kinase genes, and when using maltose as an acceptor only microorganisms that synthesis of oligosaccharides with receptor product is produced from microbes, it consists of the amino acid sequence of SEQ ID NO: 2, and sugar as a substrate The modified dextran sucrase azepin of synthesizing only a receptor oligosaccharide product is provided when using maltose as an acceptor.; Mutant strain of the present invention can be utilized in the development of a process capable of mass production of oligosaccharides, and the oligosaccharide produced from the above strain can be used as a valuable material such as medicine, food, cosmetics and the like.