Simultaneous Quantitative Analysis Method of Sphingoid Base 1-Phosphates and Sphingolipid in vivo by using o-Phthalaldehyde Precolumn Derivatization after Dephosphorylation with Alkaline Phosphatase

摘要

The present invention provides a method for simultaneously analyzing the seuping lipid 1-phosphate and seuping lipid in a number of biological samples. Specifically, after the method step of enzymatic dephosphorylation seuping lipid 1-phosphate using an alkaline phosphatase, o - phthalaldehyde (OPA) analysis method comprising the determination of the dephosphorylated seuping lipid derivative with a HPLC fluorescence assay It includes. It provides an assay method with high sensitivity and reproducibility by introducing a sphingosine 1-phosphate and, C _17- sphingosine - C ₁₇ as the internal standard. So by the methods of the present invention can analyze seuping lipid 1-phosphate and a lipid seuping from body fluids and tissues of humans and animals at the same time, carcinogenesis and metastasis (metastasis), inflammatory reactions, allergic reactions, including asthma, atherosclerosis , thrombosis, it is possible to prevent and diagnosis of heart failure, nervous system disorders, stress disorders. In another aspect, the present invention seuping lipid 1-vascular wound healing associated with the new function (angiogenesis) of the phosphoric acid, embryo formation and diagnosing organs of the body seuping playing, causing the blood to create or rheumatoid arthritis, such as diabetic retinopathy, psoriasis notice query Money can be used for sintering.