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STABILIZED AQUEOUS NUCLEOSIDE TRIPHOSPHATE SOLUTION

机译:稳定的三磷酸核苷水溶液

摘要

The invention concerns stable aqueous solutions containing one or several nucleoside triphosphates wherein the respective solution has a pH value of more than 7.5 and contains no additional substances with a stabilizing effect. The nucleoside triphosphate solutions are used in particular for DNA synthesizing reactions such as e.g. RT-PCR, cycle sequencing, random priming and nick translation. One of the most important applications of such solutions containing deoxy-nucleoside triphosphates (d-NTP) is their use in the polymerase chain reaction (PCR).
机译:本发明涉及含有一种或几种三磷酸核苷的稳定水溶液,其中各自的溶液具有大于7.5的pH值,并且不包含具有稳定作用的其他物质。核苷三磷酸酯溶液特别用于DNA合成反应,例如DNA。 RT-PCR,循环测序,随机引物和缺口翻译。此类包含脱氧核苷三磷酸(d-NTP)的溶液的最重要应用之一是它们在聚合酶链反应(PCR)中的用途。

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