Novel Recombinant Sabin Type 1 Poliovirus Vector and Improved Recombinant Polio Vaccine Composition Using the Same

摘要

PURPOSE: A recombinant Sabin type 1 poliovirus vector and a recombinant vaccine composition against poliovirus are provided, thereby inducing the formation of neutralizing antibodies against Sabin types 1, 2 and 3 polioviruses, and preventing the side-effects of OPV(attenuated oral polio vaccine; Sabin). CONSTITUTION: The recombinant Sabin type 1 poliovirus vector comprises (a) a genomic nucleotide sequence of Sabin type 1 poliovirus; (b) a nucleotide sequence encoding the additional poliovirus protease digesting site; and (c) nucleotide sequences encoding Sabin types 2 or 3 VP1 neutralizing epitopes, and connected to the nucleotide sequence encoding the additional poliovirus protease digesting site, wherein the protease is poliovirus 3C protease or poliovirus 2A protease; the nucleotide sequence encoding the Sabin type 2 VP1 neutralizing epitope is set forth in SEQ ID NO: 1; and the nucleotide sequence encoding the Sabin type 3 VP1 neutralizing epitope is set forth in SEQ ID NO: 2. The recombinant vaccine composition against poliovirus comprises (a) the recombinant Sabin type 1 poliovirus derived from the recombinant Sabin type 1 poliovirus vector; and (b) pharmaceutically acceptable carriers.