Methods of In Vitro Fertilization Showing Reduced Polyspermy and Tubes therefor

摘要

PURPOSE: A method of in vitro fertilization showing reduced polyspermy and tubes therefor are provided to reduce polyspermy while deducing a sperm penetration in a high proportion. CONSTITUTION: The method of in vitro fertilization showing reduced polyspermy comprises the steps of: disposing an ovum containing sample at the top of a tube with a sperm containing sample at the bottom; and fertilizing the ovum for a motile sperm so as to swim-up from the sperm containing sample to a location where the ovum containing sample is. The ovum and sperm are obtained from a mammal such as a pig, a cow, a sheep, a goat, a mouse, a white rat, a rabbit and a human.