METHOD early diagnosis of diseases associated with disorders of the cell genetic apparatus FUNCTIONING

摘要

1.method of early diagnosis of diseases, u0441u0432u00a0u0437u0430u043du043du044bu0445 of u0444u0443u043du043au0446u0438u043eu043du0438u0440u043eu0432u0430u043du0438u00a0 genetic apparatus of cells, including the separation of collected blood, shelter and plasma and cellular fraction of extracellular nucleic acid (u0432u043du043a)u0432u044bu00a0u0432u043bu0435u043du0438u0435 specific nucleic acid sequencing methods u0430u043cu043fu043bu0438u0444u0438u043au0430u0446u0438u043eu043du043du043eu0433u043e analysis with subsequent analysis of pcr products and the development of u0437u0430u043au043bu044eu0447u0435 u043du0438u00a0 on presence or absence u0434u0435u0442u0435u043au0442u0438u0440u0443u0435u043cu044bu0445 nucleotide sequences u043eu0442u043bu0438u0447u0430u044eu0449u0438u0439u0441u00a0, as a source of u0432u043du0435u043au043bu0435u043au0442u043eu0447u043du043eu0439 nucleic acid (u0432u043du043a) use u0432u043du043a, u0441u0432u00a0u0437u0430u043du043du044bu0435 with the surface of blood cells.the fraction of red blood cells and white blood cells in the blood cells u0440u0430u0437u0434u0435u043bu00a0u044eu0442, continue to be consistent u044du043bu044eu0438u0440u0443u044eu0442 u0432u043du043a, u0441u0432u00a0u0437u0430u043du043du044bu0435 with the cell surface of red blood cells and white blood cell response s, because of the u044du043bu044eu0430u0442u043eu0432 u0432u044bu0434u0435u043bu00a0u044eu0442 faction u0432u043du043a who use u0434u043bu00a0 u0432u044bu00a0u0432u043bu0435u043du0438u00a0 not less than two known specific sequences of nucleic acids u0441u0432u00a0u0437u0430u043du043du044bu0445 with a certain disease.;2. method for p.1, u043eu0442u043bu0438u0447u0430u044eu0449u0438u0439u0441u00a0, u044du043bu044eu0446u0438u044e u0432u043du043a, u0441u0432u00a0u0437u0430u043du043du044bu0445 with the surface of red blood cells, u043eu0441u0443u0449u0435u0441u0442u0432u043bu00a0u044eu0442 in two stages: first, u044du043bu044eu0446u0438u044e u043eu0441u0443u0449u0435u0441u0442u0432u043bu00a0u044eu0442 10 volumes of the fbi, containing 5 mm edt and in the 4u00b0u0441, followed by the deposition of cells u0446u0435u043du0442u0440u0438u0444u0443u0433u0438u0440u043eu0432u0430u043du0438u0435u043c and collecting u0441u0443u043fu0435u0440u043du0430u0442u0430u043du0442u0430, then u044du043bu044eu0446u0438u044e u043fu0440u043eu0432u043eu0434u00a0u0442 equal volume of 0.25% solution of u0442u0440u0438u043fu0441u0438u043du0430 in 4u00b0u0441 with following the addition of inhibitor enzymethe deposition of cells u0446u0435u043du0442u0440u0438u0444u0443u0433u0438u0440u043eu0432u0430u043du0438u0435u043c and collecting u0441u0443u043fu0435u0440u043du0430u0442u0430u043du0442u0430.;3. method for p.1, u043eu0442u043bu0438u0447u0430u044eu0449u0438u0439u0441u00a0, u044du043bu044eu0446u0438u044e u0432u043du043a, u0441u0432u00a0u0437u0430u043du043du044bu0445 with the count, u043eu0441u0443u0449u0435u0441u0442u0432u043bu00a0u044eu0442 in two stages: first, u044du043bu044eu0446u0438u044e u043eu0441u0443u0449u0435u0441u0442u0432u043bu00a0u044eu0442 10 volumes of the fbi, containing 5 mm u044du0434u0442u0430 in 4u00b0u0441, followed by the deposition of cells u0446u0435u043du0442u0440u0438u0444u0443u0433u0438u0440u043eu0432u0430u043du0438u0435u043c and collecting u0441u0443u043fu0435u0440u043du0430u0442u0430u043du0442u0430, then u044du043bu044eu0446u0438u044e u043fu0440u043eu0432u043eu0434u00a0u0442 equal volume of 0.125% solution of u0442u0440u0438u043fu0441u0438u043du0430 at 4c, pefc u0435u0434u0443u044eu0449u0438u043c addition of inhibitor enzymethe deposition of cells u0446u0435u043du0442u0440u0438u0444u0443u0433u0438u0440u043eu0432u0430u043du0438u0435u043c and collecting u0441u0443u043fu0435u0440u043du0430u0442u0430u043du0442u0430.;4. method for u043eu0442u043bu0438u0447u0430u044eu0449u0438u0439u0441u00a0 1, so that the fraction of u0432u043du043a u0432u044bu0434u0435u043bu00a0u044eu0442 mainly through u043cu0435u0448u0438u0440u043eu0432u0430u043du043du043eu0433u043e u043cu0435u043bu043au043eu0434u0438u0441u043fu0435u0440u0441u0442u043du043eu0433u043e glass in the presence of u0445u0430u043eu0442u0440u043eu043fu043du044bu0445 salts.;5. method for u043eu0442u043bu0438u0447u0430u044eu0449u0438u0439u0441u00a0 1, so that a u0430u043cu043fu043bu0438u0444u0438u043au0430u0446u0438u043eu043du043du044bu0445 methods use u043fu043eu043bu0438u043cu0435u0440u0430u0437u043du0443u044e chain reaction, u043bu0438u0433u0430u0437u043du0443u044e chain reaction, u043cu0443u043bu044cu0442u0438u043fu043bu0435u043au0441u043du0443u044e pcr.;6. method for 1, u043eu0442u043bu0438u0447u0430u044eu0449u0438u0439u0441u00a0, mainly u043eu0441u0443u0449u0435u0441u0442u0432u043bu00a0u044eu0442 early diagnostic level and pathologies of pregnancy.