METHOD FOR COMPREHENSIVE PROCESSING OF BLOOD OF AGRICULTURAL ANIMALS FOR OBTAINING BIOLOGICALLY ACTIVE SUBSTANCE WITH ANTI-ANEMIC PROPERTIES BASED ON HEMOGLOBIN, BIOLOGICALLY ACTIVE SUBSTANCE RESOURCES

摘要

FIELD: technology for preparing biologically active compounds from animal raw. ^ SUBSTANCE: invention relates to industrial method for isolation of iron-containing protein - hemoglobin from blood of agricultural animals. Raw for preparing hemoglobin represents erythrocyte mass from stabilized whole blood, blood cells or nonstabilized coagulated whole blood that is finely milled preliminary to particles size 1-5 mm. After preliminary destruction of erythrocytes the raw is diluted successively with distilled water for extraction of hemoglobin and alleviating separation of erythrocyte membranes, fibrin and stroma. Hemoglobin solution is prepared by using a biphase system for separation of erythrocyte mass hemolyzate obtained by its successive dilution with distilled water with addition of 1-3% of sodium chloride in the ratio = (0.1-2):1 of raw volume and at maintaining pH value of solution in the range 5.5-8 at temperature 4-10C. After successive carrying out the first, second and third extraction and further purification by using membrane macro-, micro- and ultrafiltration and ion-exchange chromatography, stabilization and drying by using low-temperature spraying or sublimating drying the end products are prepared with the enhanced yield of hemoglobin up to 98% comprising from 60 to 99% of hemoglobin with the content of iron from 0.16 to 0.3% in reduced form. Hemoglobin of different purity degree can be used as a concentrated natural source of biologically available iron in manufacturing functional foodstuffs, biologically active supplements, fodders, veterinary and medicinal preparations used in prophylaxis and treatment of iron-deficient states in humans and animals. Proposed group of inventions promotes to simplifying and reducing cost of the process in industrial preparing blood hemoglobin from agricultural animals with simultaneous improvement of quality and its enhanced yield. ^ EFFECT: improved processing and preparing method. ^ 25 cl, 2 dwg, 9 tbl, 5 ex