The new enzyme, the peptides, microorganisms producing the enzyme, and the methods for synthesizing dipeptides THEIR USE

摘要

1. the enzyme obtained from a microorganism, u043eu0442u043du043eu0441u00a0u0449u0435u0433u043eu0441u00a0 to the family, a kind of Empedobacter and kind of Sphingobacterium, having the ability to form peptide of u043au0430u0440u0431u043eu043au0441u0438u043b u044cu043du043eu0433u043e component and u0430u043cu0438u043du043du043eu0433u043e component.;2. the enzyme with the ability to form peptide of u043au0430u0440u0431u043eu043au0441u0438u043bu044cu043du043eu0433u043e component and u0430u043cu0438u043du043du043eu0433u043e component and the ability to form the u0430u043bu0430u043du0438u043b - l - glutamine with soon. stu u043eu0431u0440u0430u0437u043eu0432u0430u043du0438u00a0 0.03 mm / min or above in the reaction u043eu0431u0440u0430u0437u043eu0432u0430u043du0438u00a0 u0434u0438u043fu0435u043fu0442u0438u0434u0430 in u0443u0441u043bu043eu0432u0438u00a0u0445 (i) - (iv).;(i) u043au0430u0440u0431u043eu043au0441u0438u043bu044cu043du044bu043c component u00a0u0432u043bu00a0u0435u0442u0441u00a0 hydrochloride complex u043cu0435u0442u0438u043bu043eu0432u043eu0433u043e ether l - u0430u043bu0430u043du0438u043du0430 of 100 mm.;(ii) u0430u043cu0438u043du043du044bu043c component u00a0u0432u043bu00a0u0435u0442u0441u00a0 l - glutamine in the amount of 200 mm;;(iii) the same ph 9.0.;(iv) the number of u0434u043eu0431u0430u0432u043bu00a0u0435u043cu043eu0433u043e u0433u043eu043cu043eu0433u0435u043du043du043e purified enzyme u0441u043eu0441u0442u0430u0432u043bu00a0u0435u0442 less 0.61 mg / ml per protein.;3. enzyme for 1 or 2, which u043au0430u0440u0431u043eu043au0441u0438u043bu044cu043du044bu0439 component as a substrate includes both complex ether amino acids, and amide amino acids.;4. enzyme for 1 or 2, which u0430u043cu0438u043du043du044bu0439 component as a substrate includes any of the amino acid, c is a protected amino acid and amine.;5. enzyme for 1 or 2, in which the enzyme has the ability to form peptide in the range of ph from 6.5 to 10.5.;6. enzyme for 1 or 2, in which the enzyme has the ability to form peptide in the temperature ranges from 0 to 60C.;7. enzyme for 1 or 2, in which the enzyme is not u0438u043du0433u0438u0431u0438u0440u0443u0435u0442u0441u00a0 u0441u0435u0440u0438u043du043eu0432u043eu0433u043e enzyme inhibitor, u0444u0435u043du0438u043bu043cu0435u0442u0438u043bu0441u0443u043bu044cu0444u043eu043du0438u043bu0444u0442u043eu0440u0438u0434u043eu043c, but u0438u043du0433u0438u0431u0438u0440u0443u0435u0442u0441u00a0 couple - u043du0438u0442u0440u043eu0444u0435u043du0438u043b - a '- u0433u0443u0430u043du0438u0434 u0438u043du043eu0431u0435u043du0437u043eu0430u0442u043eu043c.;8. enzyme for 1 or 2, in which the enzyme is u043cu043eu043bu0435u043au0443u043bu00a0u0440u043du0443u044e mass determined by sds - gel electrophoresis, u0441u043eu0441u0442u0430u0432u043bu00a0u044eu0449u0443u044e approximately 75 u043au0438u043bu043eu0434u0430u043bu044cu0442u043eu043d and u043cu043eu043bu0435u043au0443u043bu00a0u0440u043du0443 yu mass determined by chromatography using gel filtration, u0441u043eu0441u0442u0430u0432u043bu00a0u044eu0449u0443u044e approximately 150 u043au0438u043bu043eu0434u0430u043bu044cu0442u043eu043d.;9. micro organism which produced the enzyme in 1 or 2.;10. a microorganism for p.9, which a microorganism selected from the Empedobacter brevis, strain FERM bp - 8113 and Sphingobacterium from strain FERM bp - 8124.;11. method u043fu043eu043bu0443u0447u0435u043du0438u00a0 u0434u0438u043fu0435u043fu0442u0438u0434u0430, including u0441u0435u0431u00a0 education u0434u0438u043fu0435u043fu0442u0438u0434u0430 of u043au0430u0440u0431u043eu043au0441u0438u043bu044cu043du043eu0433u043e component and u0430u043cu0438u043du043du043eu0433u043e component using the enzyme for 1 or 2 or the substance containing the enzyme.