HOMODIMERIC FUSED PROTEIN WITH ACTIVITY OF ANGIOGENESIS INHIBITOR (VARIANTS), DNA MOLECULE ENCODING HOMODIMERIC FUSED PROTEIN, VECTOR FOR EXPRESSION OF HOMODIMERIC FUSED PROTEIN, METHOD FOR TRANSFECTION OF MAMMALIAN CELL AND METHOD FOR PREPARING HOMODIMERIC FUSED PROTEIN

摘要

FIELD: biotechnology, molecular biology.;SUBSTANCE: invention can be used for preparing fused proteins as inhibitors of angiogenesis. Fused protein represents a homodimer wherein region Fc in immunoglobulin-gamma is fused with the target protein comprising one or some molecules with activity of angiogenesis inhibitor that originates from angiostatin or endostatin. Variant of homodimeric fused protein involves also the second target protein comprising one or some molecules eliciting activity of angiogenesis inhibitor and taken among angiostatin, endostatin, plasminogen fragment eliciting activity of angiostatin, and collagen XVIII fragment eliciting activity of endostatin. Molecules as components of homodimeric used protein are bound with region Fc in immunoglobulin-gamma and each with other directly or by a polypeptide bridge. Homodimeric fused protein is prepared by transfection of mammalian cell with vector comprising DNA molecule encoding the target protein, culturing mammalian cells for producing fused protein and isolation of fused protein. Invention provides effective production and secretion of agiogenesis inhibitors in different hosts - mammalian cells.;EFFECT: improved preparing method, valuable biological and medicinal properties of protein.;13 cl, 6 dwg, 16 ex